Mifune, too, was a staple, having been discovered by the director after throwing a fit after getting in the wrong queue for a job at Toho Studios, and had starred in six prior films for the director, including “ Rashomon.” Some of the actors had curious backstories, however. Takashi Shimura, who plays leader Kambei Shimada, was a long-time favorite of Kurosawa‘s and had starred in “ Ikiru” two years earlier for the director. Some of the cast, particularly the leads, were no-brainers. Like the characters, the cast came from a diverse range of sources. As a result, they created the pretender samurai Kikuchiyo, who’s more empathetic and emotional, and cast Toshiro Mifune, who was originally going to play badass swordsman Kyuzo (later taken by Seiji Miyaguchi), in the new role, giving the actor free rein to improvise.Ģ. The 500-page first draft (co-written with regular collaborators Hideo Oguni and Shinobu Hashimoto) was titled “ Six Samurai,” but on reflection, they found that having six responsible, sober samurai heroes was a little dry. Instead, he decided to adapt a story he’d found about a group of Samurai defending a village of farmers. His original conception for the project was to follow the day in the life of a samurai, ending with the protagonist’s suicide, but he wasn’t confident enough in the level of research he’d need to do. Originally, the film was going to be called “Six Samurai”Īlthough it’s now the genre with which he’s most associated, Kurosawa had never made a samurai movie before this point. Following a personal breakdown in the late sixties, Kurosawa rebounded by expanding his dark brand of humanism into new stylistic territory, with films such as Kagemusha and Ran, visionary, color, epic ruminations on modern man and nature.‘Living’ Teaser: Bill Nighy Leads British Reimagining of Akira Kurosawa’s ‘Ikiru’ġ. Kurosawa would subsequently gain international fame with Rashomon, a breakthrough in nonlinear narrative and sumptuous visuals. The first serious phase of Kurosawa’s career came during the postwar era, with Drunken Angel and Stray Dog, gritty dramas about people on the margins of society that featured the first notable appearances by Toshiro Mifune, the director’s longtime leading man. His best-known films remain his samurai epics Seven Samurai and Yojimbo, but his intimate dramas, such as Ikiru and High and Low, are just as searing.
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2017 7:215.īerry J-L, Gurung I, Anonsen JH, Spielman I, Harper E, Hall AMJ, et al. The type IX secretion system (T9SS): highlights and recent insights into its structure and function. Lasica AM, Ksiazek M, Madej M, Potempa J. Type IV pili of Streptococcus sanguinis contribute to pathogenesis in experimental infective endocarditis. Martini AM, Moricz BS, Woods LJ, Jones BD. Functional analysis of an unusual type IV pilus in the Gram-positive Streptococcus sanguinis. Gurung I, Spielman I, Davies MR, Lala R, Gaustad P, Biais N, et al. Molecular and functional analysis of the type IV pilus gene cluster in Streptococcus sanguinis SK36. Identification of genes and genomic islands correlated with high pathogenicity in Streptococcus suis using whole genome tiling microarrays. Zheng X, Zheng H, Lan R, Ye C, Wang Y, Zhang J, et al. Catabolite control protein A controls hydrogen peroxide production and cell death in Streptococcus sanguinis. Zheng L, Chen Z, Itzek A, Ashby M, Kreth J. Murein hydrolase LytF of Streptococcus sanguinis and the ecological consequences of competence development. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press 1989.Ĭullin N, Redanz S, Lampi KJ, Merritt J, Kreth J. Evaluation of phenotypic and functional stability of RAW 264.7 cell line through serial passages. Taciak B, Białasek M, Braniewska A, Sas Z, Sawicka P, Kiraga Ł, et al. The cia operon of Streptococcus mutans encodes a unique component required for calcium-mediated autoregulation. He X, Wu C, Yarbrough D, Sim L, Niu G, Merritt J, et al. Chemically defined medium for oral microorganisms. Live and let die: hydrogen peroxide production by the commensal flora and its role in maintaining a symbiotic microbiome. Redanz S, Cheng X, Giacaman RA, Pfeifer CS, Merritt J, Kreth J. Corncob structures in dental plaque reveal microhabitat taxon specificity. Morillo-Lopez V, Sjaarda A, Islam I, Borisy GG, Mark, Welch JL. Synergism between Corynebacterium and Streptococcus sanguinis reveals new interactions between oral commensals. Treerat P, Redanz U, Redanz S, Giacaman RA, Merritt J, Kreth J. Role of microbial communities in the pathogenesis of periodontal diseases and caries. The oral host-microbial interactome: an ecological chronometer of health? Trends Microbiol. Biogeography of a human oral microbiome at the micron scale. Mark Welch JL, Rossetti BJ, Rieken CW, Dewhirst FE, Borisy GG. Biogeography of the oral microbiome: the site-specialist hypothesis. Obligate cross-feeding expands the metabolic niche of bacteria. Ona L, Giri S, Avermann N, Kreienbaum M, Thormann KM, Kost C. The impact of horizontal gene transfer on the adaptive ability of the human oral microbiome. Co-ordinated bacteriocin production and competence development: a possible mechanism for taking up DNA from neighbouring species. Oral microbial communities: genomic inquiry and interspecies communication. Antagonistic, synergistic, and counteroffensive strategies for streptococcal interspecies interactions. Hutchinson’s duality: the once and future niche. Reduced twitching motility could lead to a closer interaction of both species, supporting niche development in the oral cavity and potentially shaping symbiotic health-associated biofilm communities.Ĭolwell RK, Rangel TF. durum produced glycerol is crucial for the interaction of C. In conclusion, we identified that the ability to metabolize C. durum, which was caused by a decrease in type IV pili abundance on the surface of S. sanguinis twitching motility in the presence of C. Investigations on the effect of type IV pili revealed a reduction of S. durum secreted glycerol and impaired chain elongation important for their interaction. Gene inactivation of the glycerol kinase glpK had a profound impact on the ability of S. We further explored the differentially expressed type IV pili genes due to their connection to motility and biofilm adhesion. Due to its connection to fatty acid synthesis, we focused on the glycerol-operon. Coculture experiments for transcriptomic analysis identified several differentially expressed genes in S. Here we present the molecular mechanisms of this interspecies interaction. sanguinis chain elongation and overall increased fitness supporting their commensal state. We previously reported that Streptococcus sanguinis and Corynebacterium durum have a close relationship through the production of membrane vesicle and fatty acids leading to S. To understand what molecular mechanisms determine these interactions, we used information gained from recent biogeographical investigations demonstrating an association of corynebacteria with streptococci. During oral biofilm development, interspecies interactions drive species distribution and biofilm architecture. After making the adjustment, turn the water to the tank back on by screwing the handle on the water input hose to the left. With the float in the proper position, tighten the clip by either releasing it or rotating it. Adjust the float until it is even with the water line that you identified earlier.Ħ. Lower or raise the float by pushing down on it or pulling up on it. Loosen the clip by either squeezing or rotating it.ĥ. Looking into the tank, you'll see a long tube that runs from the float to the fill valve. If it's too high or too low, you'll need to repeat the process to make the appropriate adjustment.ģ. Once it's full, make sure that the float is sitting at the water level. After making the adjustment, turn the water to the tank back on by turning the handle on the water input hose to the left. You can loosen the screws more to lower the float, or you can tighten them to raise the float.ĥ. Looking at the water line on the tank, determine where the float needs to sit. The screws are often located close to the fill valve.Ĥ. Using a screwdriver, loosen the screws holding the float in place by turning counterclockwise. Turn that handle all the way to the right until it's tight.ģ. That hose goes into your wall or floor, and there will be a handle at the base of the hose. Your toilet's water supply comes through a hose that's often on the left side of the toilet. Make reference of this point so you know where you'll want your float to sit after the adjustment.Ģ. There might be a line on the toilet tank that identifies the ideal water line. It's best to have your float set so it is one to two inches beneath the overflow tube. Adjust the chain so there's about ½-inch of slack when your flush lever is in the normal position.ġ. If it's too short, the flapper may not be able to close and seal properly. If it's too long, the flapper won't fully lift off of the drain hole, so you won't get a full flush. The flapper chain needs to be the right length. The exact installation method you'll use will depend on the type of flapper you have, so refer to the directions that come with your new flapper.Ħ. Most hardware store staff will also be able to help you.ĥ. The easiest way to ensure you're buying the right flapper is to take your old flapper to the hardware store with you so you can compare the models. Your new flapper will need to be the same size and type as your old one. Different flappers mount into the tank in different ways, but you should be able to wiggle, bend, and otherwise remove the flapper from the base of the tank.Ĥ. The flapper will have a chain that attaches to the flush lever inside your tank, which you can unclip. By flushing the toilet, you'll drain all of the remaining water from the tank.ģ. Turn that handle all the way to the right until it's tight.Ģ. If you've determined that the flapper is leaking, then you'll need to replace it. The flapper is at the base of the tank and lets water flow from the tank to the bowl. It highly supports any frequently-used video/audio format. If you want to play common videos and Blu-rays on your Windows PC or Mac, this powerful QuickTime Player alternative can handle the task. QuickTime alternative Best alternative for QuickTime Playerīlu-ray Player is the best alternative to QuickTime Player.
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It was the second consecutive relief appearance in which Morris did not allow a run. Morris stranded the bases loaded when pinch hitter Connor Hujsak lined out to left field on the final pitch he threw. With another sweep, the Razorbacks would tie the program record set by Arkansas’ 1999 SEC championship team that swept five times.Īrkansas left-hander Zack Morris threw three innings of scoreless relief Sunday in his most efficient outing of the season.Īfter inheriting a base runner in the sixth inning, Morris induced two ground-ball outs - including an inning-ending double play - and retired seven consecutive batters until Mississippi State loaded the bases on a hit batsman, an error and a walk in the eighth. It's something that if you have a chance to get it and you can get it, you check the standings at the end of the year and you've jumped in front of a few people." "It's hard beating them three times anywhere. "It's hard beating anybody three times on the road," Van Horn said before Sunday's game on the Razorback Sports Network. Arkansas has not swept anywhere more than Mississippi State (3) under Van Horn. Under 21st-year coach Dave Van Horn, the Razorbacks have 39 SEC sweeps, including 10 away from home. The Crimson Tide played their home games at Hoover (Ala.) Metropolitan Stadium that season while their on-campus stadium in Tuscaloosa was being renovated.Īside from Mississippi State, Arkansas has not swept on an SEC campus since 2014 at Missouri. Since 2016, the Razorbacks have swept a team on the road twice - both times at Mississippi State.Īrkansas also swept at Mississippi State’s Dudy Noble Field in 2021 when the Bulldogs won the national championship.īefore that, the Razorbacks’ last SEC sweep away from home came in 2015 over Alabama. All of the 2018 sweeps were in series played at home. The four sweeps are the program’s most in SEC play since the 2018 Razorbacks also swept four teams. The sweeps are a big reason why the Razorbacks’ 24-game SEC record is the same as the 2021 Arkansas team that won the SEC with a 22-8 record. Ten of his career homers have come with runners on base, including two grand slams and 6 three-run home runs.Īrkansas has swept four SEC teams - Auburn, Tennessee, Texas A&M and Mississippi State - as part of a 17-7 start to SEC play.Īrkansas is tied with Vanderbilt for the best record in the SEC with two weeks remaining in the regular season. Both came as part of a five-run inning.ĭiggs hit a two-run home run during the top of the seventh inning Saturday when the Razorbacks won 14-2 in seven innings.ĭiggs’ 13 career home runs have accounted for 33 RBI. The grand slam was Diggs’ second home run of the series. “ see a pitch up in the zone and really getting my 'A' swing off every time.” “I’ve just been evaluating my swing, how I’m feeling, looking back at my approach, talking with the coaches - just really focusing on staying through the ball,” Diggs said following Saturday’s game. Three days before the Mississippi State series began, Diggs went 0 for 5 during an extra-innings loss to Lipscomb at Dickey-Stephens Park in North Little Rock. It appears Diggs rebounded from a rough patch when he went hitless in four of five games. He and Jace Bohrofen each had 4 RBI while hitting in the top spots in the batting order. 643 on-base percentage during the weekend and led off each game with either a walk or a hit.ĭiggs hit a grand slam during the fifth inning of the Razorbacks’ 11-6 victory over the Bulldogs on Sunday to complete a three-game sweep. Kendall Diggs might have a player of the week award awaiting him Monday.īatting leadoff, the Arkansas right fielder finished the series at Mississippi State 6 for 11 with 3 walks, 10 RBI and 5 runs scored. yes alot of people use it but that's because they go with the company that provides them with intelligent lighting. What limitations does freestyler have? i use both freestyler and light jockey and its known fact that that freestyler can do more than light jockey can.Īnd light jockey is far from industry standard. you'll get help on the freestyler forums faster than you will calling or emailing martin tech support. LJ can be fully programmed to run your show with few input from the dj. It has a step learning curve but as a Martin Product is super solid and is the stepping stone for the big lighting leagues like GrandMA, avolites and Hog that are used in concerts and Arenas. LJ is U$14000 but is amazing and like Serato is the industry standard. In a way is a incomplete copy of LJ but it serve me well. Is an unsupported software with a lot of limitations. Freestyler with Entec is a good way to start but is limited. Try to make a circle and you will agree with me. For a cheap controller is better to run the lights Master-slave. No notion of cue buttons (recall multiple scenes), no notion of override (override currently playing scene) and no notion of submasters/subcues (mix and match different parts of different scenes on the fly).Īnd the fact that no 3D until (let's just say 2014) is wack. and you can only run one scene at a time! basically you can only create a scene with x number of steps. The scene builder is ok, but the scene system is extremely limited. alot of space is wasted with the dmx faders. especially when you have a lot of lights. you can't create/edit fixtures on the mac. I've tried myDMX, lightjockey and showexpress. recently i started getting into the midi mapping and submasters. The reason that i love it is because it is free, works with any interface and has some extremely powerful features. Have you tried running it virtually on your mac? i'm not sure what version of FreeStyler you are using or the system that it is running on, but ever since i have started using it a few years ago it has been nothing but flawless for me. i wouldn't recommend it for programming a light show with various fixtures. Makes sense because it is for controlling lasers. While Pangolin does DMX it is missing some key features that any DMX software should have. |